中英文说明书丨Abbkine 总蛋白提取试剂盒解决方案

Abbkine 总蛋白提取试剂盒英文说明书：

Background

Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity.

Specification

Product name ExKine™ Total Protein Extraction Kit

Applications notes Abbkine ExKine™ Total Protein Extraction Kit is composed of rapid and efficient solutions for lysis of mammalian adherent cells, nonadherent cells, and tissues, which is compatible with many different applications, such as reporter assays, protein purification, Western blot, immunoprecipitation.

Product Properties

Kit components • Protein Extraction Reagent (2×)

• Protease Inhibitor (100×)

Usage notes Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.

Storage instructions Stable for at least 12 months at -20°C from date of shipment.

Shipping Gel pack with blue ice.

publications

MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression

Author：J Sun, Q Ma, C Shu Publication name：International Journal of Oncology IF：7.1

Abbkine 总蛋白提取试剂盒中文说明书：

背景：

细胞蛋白的提取需要有效的细胞裂解和蛋白质溶解，同时避免蛋白质降解和/或干扰蛋白质免疫反应性和生物活性。

实验建议：

Abbkine ExKine™ 总蛋白提取试剂盒适用于从哺乳动物组织和培养细胞（贴壁或悬浮细胞）中提取全蛋白，配套有蛋白酶抑制剂Cocktail，作用温和，提取过程简便高效。

试剂盒组分：

• 蛋白提取试剂 (5X)

• 蛋白酶抑制剂 (100×)

使用中注意事项：在2-8°C下执行所有步骤。 使用预冷的缓冲液和设备。 确保所有解决方案都是解冻和均匀的。

保存建议 建议保存于-20°C。

运输条件 蓝冰运输

*仅供研究使用，不适用于人类或临床诊断。

文献引用：

MicroRNA‑301a/ZNRF3/wnt/β‑catenin signal regulatory crosstalk mediates glioma progression

作者：J Sun, Q Ma, C Shu 杂志名称：International Journal of Oncology IF：7.1